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Advances in Biochemical Engineering Biotechnology Metabolic by Georg Gübitz, Alexander Bauer, Guenther Bochmann, Andreas

By Georg Gübitz, Alexander Bauer, Guenther Bochmann, Andreas Gronauer, Stefan Weiss

Michael Lebuhn, Stefan Weiß, Bernhard Munk, Georg M. Guebitz

Microbiology and Molecular Biology instruments for Biogas method research, analysis and Control

Veronika Dollhofer, Sabine Marie Podmirseg, Tony Martin Callaghan, Gareth Wyn Griffith & Katerina Fliegerová

Anaerobic Fungi and their strength for Biogas Production

Bianca Fröschle, Monika Heiermann, Michael Lebuhn, Ute Messelhäusser, Matthias Plöchl

Hygiene and Sanitation in Biogas Plants

Charles-David Dubé and Serge R. Guiot

Direct Interspecies Electron move in Anaerobic Digestion: A Review

Simon K.-M. R. Rittmann

A severe review of Microbiological Biogas to Biomethane Upgrading Systems

Manfred Lübken, Pascal Kosse, Konrad Koch, Tito Gehring, Marc Wichern

Influent Fractionation for Modeling non-stop Anaerobic Digestion Processes

Fermoso, F. G, van Hullebusch, E. D, Guibaud, G, Collins, G,  Svensson, B. H, Carliell-Marquet, C, Vink, J.P.M,  Esposito, G,  Frunzo, L

Fate of hint Metals in Anaerobic Digestion

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Strobel RJ, Nakatsukasa WM (1993) Response surface methods for optimizing Saccharopolyspora spinosa, a novel macrolide producer. J Ind Microbiol 11:121–127 26. Boeck LD, Favret ME, Wetzel RW (1992) Biosynthesis of thiopeptide antibiotic A10255 in stirred reactors using a chemically defined medium supplemented with continuous nutrient feeds. J Antibiot 45:1278–1285 27. Hosler P, Johnson MJ (1953) Penicillin from chemically defined media. Ind Eng Chem 45:871–874 28. Jain D, Nielson JBK, Buckland BC (1992) Kinetics of efrotomycin synthesis in a synthetic fermentation medium.

34 5 Classical Strain Improvement for Polyketide Production . . . 34 6 Process Development for Polyketide Production . . . . . . 3 Clone Selection and Culture Preservation . . . . . . . . 35 Media Development . . . . . . . . . . . . . . 36 Small-Scale Fermentation . . . . . . . . . . . . 37 7 Metabolic Engineering for the Overproduction of Polyketides . . 5 Heterologous Expression of Polyketides . . . . Maximizing Gene Expression . . . . . . .

Polyketides are biosynthesized by large multi-enzyme systems called polyketide synthases (PKSs). These large synthases, which are modular in architecture and function, catalyze step-wise elongation of a polyketide chain, as well as associated functional group modifications. At each step in the chain elongation process, an acyl group monomer is recruited from the available metabolic pool of acyl-CoA precursors. Typical precursors include metabolites such as acetylCoA, propionyl-CoA, and other alkyl-CoAs, which are used as chain initiators, and malonyl-CoA and methylmalonyl-CoA, which are used for the elongation process.

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